random primer造句
例句與造句
- Three natural population of l . sagitta , add up to 62 individual , with 17 random primers , 128 polymorphic loci
箭葉橐吾的三個自然居群，總計62個個體，使用17個隨機引物，共擴增出128個多態(tài)位點。 - Five natural population of l . virgaurea , add up to 100 individual , with 17 random primers , 132 polymorphic loci
黃帚橐吾的五個自然居群，總計100個個體，使用17個隨機引物，共擴增出132個多態(tài)位點。 - Rapd technique was first used to analyze the phylogenetic relationships of 13 species or varieties in pueraria dc complex . seventy - two random primers were tested , among which 18 chosen primers generated clear polymorphic bands
從72個隨機引物中篩選出了18個引物在13個不同的種和類型的葛資源中具有rapd多態(tài)性。 - Using 20 out of 40 decamer random primers , random amplified polymorphic dna ( rapd ) was applied to analyze the diversity of 48 individuals of p . polyactis from five sampling areas in the yellow sea and the east china sea
研究主要包括如下三個部分：一、小黃魚的遺傳多樣性分析了采自黃海和東海5個取樣海區(qū)共計4呂個小黃魚個體的隨機擴增dna多態(tài)性。 - Twelve primers selected from 100 random primers amplified 254 frangments in 14 species of cymbidium , in which 243 segments are polymorphic . the rate of polymorphism was 95 . 7 % . and all the segments were ranging from 200bp to 2200bp
從100個隨機引物中篩選出12個隨機引物，對蘭屬植物14種材料進行隨機擴增，共得到254條帶，多態(tài)性帶有243條，多態(tài)性百分率為95 . 7 。 - It's difficult to find random primer in a sentence. 用random primer造句挺難的
- Finally , the variant line tc243 - 2 , was analyzed by rapd - pcr amplification with 100 random primers . the results suggested that phenotypic variation came from exogenous dna transformation rather than exogenous pollen contamination . a mutation corre
用100條隨機引物對tc243一株系的rapd一pcr擴增結(jié)果表明該株系的變異不是來自外源花粉的污染，同時發(fā)現(xiàn)了特異條帶5270 _ 1600對應的變異在tz一ts代能連續(xù)穩(wěn)定遺傳。 - Random amplified polymorphic dna ( rapd ) analysis demonstrated that 16 random primers produced 129 loci , obtaining 128 polymorphic loci in 7 populations from different geographical locations . the total percentage of polymorphism was 99 . 22 % , which showed s . krylovii had abundant genetic diversity
采用rapd方法對不同地理種群的研究表明， 16個引物共擴增出129個位點，其中多態(tài)位點128個，多態(tài)位點百分率是99 . 22 ，說明克氏針茅具有較高的遺傳多樣性。 - Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最后，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結(jié)合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。 - Similarity coefficient and genetic distances between two arbitrary individuals were caculated according to the data of experiments , the pedigree was constructed by two means ( they were upgma and parsimony ) , and genetic diversity was discussedbased on selecting the best conditions of pcr with opg and opa primers from operon company , we obtained 163 bands using 24 random primers . in other words , 163 sites in crested ibis " genome were detected . the bands from 23 primers showed polymorphism . minimurn of polymorphic ratio was 0 , maximum was 85 . 71 % , and average was 48 . 99 % . in the population which was composed by 37 individuals , most of genetic distances between two arbitrary individuals were less than 0 . 1 . the results showed that the degree of similarity among crested ibises was high while genetic diversity in the population was low accordingly
我們在對operon公司opg系列和opa系列引物進行優(yōu)化篩選的基礎上，用24條隨機引物共擴增出163條譜帶，即檢測了朱?基因組中的163個位點，其中23條引物的擴增結(jié)果具有多態(tài)性；譜帶的多態(tài)比率最小為0 ，最大為85 . 71 ，平均為48 . 99 ；在由37只朱?組成的種群中，任意兩個個體之間的遺傳距離絕大多數(shù)在0 . 1以下。這些結(jié)果表明，目前朱?個體間的相似程度很高，種群的遺傳多樣性水平較低。 - Abstract : to decrease nonspecific products and obtain clear rapd patterns , 18 10 - mer random primers were tested at different annealing temperatures . the results indicated that all the amplification can be performed when the annealing temperature is in the range of 40 ~ 50 . there were a few primers with which the amplification was still performed when the annealing temperature is above 60 . by using high annealing temperatures , some primers which produce more nonspecific product at the annealing temperatures of 35 ~ 38 could generate reproducible and distinct bands
摘要用38 66不同復性溫度處理,比較了18條隨機引物的擴增結(jié)果.發(fā)現(xiàn)復性溫度在40 50之間均有數(shù)量不等的擴增產(chǎn)物,不同引物的最高復性溫度不同,有些引物用60以上的復性溫度仍有擴增產(chǎn)物.一些在35 38的復性溫度下非特異性產(chǎn)物較多的引物,通過大幅度提高復性溫度,能提高擴增產(chǎn)物的特異性,獲得清晰的rapd帶型。 - Four out of 150 random primers could detect dna polymorphism between the two mutants and the wild type arabidopsis thaliana , which provided a strong evidence for the truth of the mutants . only one primer gave a specific dna fragment about 1200bp which two mutants own but the parent do n ' t . we drew a preliminary conclusion that the fragment might be related to the salt - tolerance gene
擬南芥抗鹽突變體的rapd分析結(jié)果表明， 150條引物中有四條引物在突變體和野生型擬南芥之間擴增出多態(tài)性，證明了dna水平突變的發(fā)生，其中1條引物在突變體的擴增產(chǎn)物比在野生型的擴增產(chǎn)物多出一個大小約為1200bp的片段，初步認為該片段可能與植物的抗鹽性有關。 - And the genome dna of seven strains of spirulina platensis as mentioned above are analyzed with 106 random primers using random amplified polymorphic dna ( rapd ) technique . with the 106 random primers , 23 primers revealed their obvious polymorphisms and generated a total of 151 fragments , 120 of which were polymorphic
在此基礎上，采用優(yōu)化后的提取方法制備sp - d等7株鈍頂螺旋藻的基因組dna ，利用106條隨機引物對其進行rapd擴增，從中篩選出23條多態(tài)性引物，其擴增出的總條帶數(shù)為151 ，多態(tài)性條帶數(shù)為120 。 - 2 . a total of 88 loci ( including 65 polymorphic loci ) were amplified using 13 issr primers for issr and a total of 97 loci ( including 66 polymorphic loci ) were amplified using 12 random primers for rapd . the average percentage of polymorphic bands ( ppbs ) are 71 . 6 % for issr and 68 % for rapd respectively
用13個issr引物和12個rapd引物分別對6個群體共90個銀杏dna樣品進行擴增，分別得到88個(其中多態(tài)性位點65個)和97個(其中多態(tài)性位點66個)清晰的擴增位點，多態(tài)性位點百分數(shù)( ppb )分別為71 . 6和68 。 - It may suggest that h . patula had different genomes from leymus , psathyrostachys and the other three species of hystrix in this study . 6 . comparison between the results from rapd and those from issr , it is concluded that : ( 1 ) 40 random primers were screened for rapd fragments , 20 primers produced polymorphic rapd products
（ 2 ）結(jié)果分析的比較：通過兩種不同dna分于標記得出的伽tris 、 lcymus和psathmptachys三個屬間的jaccard遺傳相似系數(shù)及遺傳聚類圖具有極顯著的相關性，表明研究中所用的rapd和issr標記數(shù)量可以檢測到這些遺傳變異。